DEPARTMENT:

Basic Sciences


CAMPUS AFFILIATION:

Doylestown


OFFICE:

Geisinger Commonwealth School of Medicine
Doylestown Campus
Pennsylvania Biotechnology Center of Bucks County
3805 Old Easton Road, Office 307
Doylestown, PA 18902


PHONE:

267-423-7152



EDUCATION:

PhD in Biochemistry – Ewha Womans University, Seoul, South Korea, 2010, Thesis title: Characterization of the biosynthetic route to 4,6-disubstituted aminoglycosides using a heterologous expression system   

MS in Biochemistry – Ewha Womans University, Seoul, South Korea, 2005, Thesis title: Dimyristoylphosphatidylcholine-induced modification of lipid rafts in human erythrocytes

BS in Chemistry – Ewha Womans University, Seoul, South Korea, 2003


BIO:

Sung Ryeol Park, PhD’s background is in microbial biochemistry and genetic engineering. In her PhD, she developed a heterologous expression system for natural product pathways using the terrestrial microbe. The system aimed to improve the production yield of secondary metabolites. She also leveraged the established heterologous expression system to better understand the biosynthesis of aminoglycoside antibiotics.

After completing her doctoral degree, Dr. Park moved to University of Michigan as a postdoctoral fellow. During that time, she led multiple projects that span a variety of biosynthetic enzyme classes including polyketide synthases, nonribosomal pepetide synthetases, methyltransferases and cytochrome P450s. She also biochemically characterized several enzymes from different natural product biosynthetic pathways and is actively exploring the substrate scope and biocatalytic potential of these enzymes. In addition, she generated microorganism-based biotransformants in which a native cytochrome P450 is replaced by multiple P450 variants capable of selectively oxidizing C-H bonds in non-native substrates.

Dr. Park’s time during PhD and postdoctoral fellowship inspired a strong desire to expand her research scope to include diverse natural product biosynthetic systems that are ideal for discovery of promising lead compounds. This led her to the natural products discovery institute (NPDI), a division of Baruch S. Blumberg Institute, as a senior microbiologist in 2015, where she was appointed as an assistant professor in 2016. Dr. Park currently applies her expertise and experiences in bacterial culture/fermentation and characterization of biochemical pathways of microorganisms derived from the vast NPDI collection.

Dr. Park’s professional experiences and deep understanding of the biochemistry, genetics and enzymology of microbial natural product biosynthesis and the fermentation process enable her to contribute to the screening and optimization of biologically active molecules. Moreover, her research will attract scientists and pharmaceutical societies from biology, chemistry, applied microbiology and bioinformatics to develop novel tools and strategies for the discovery of new potential drug leads.


EDUCATIONAL INTERESTS:

Sung Ryeol Park, PhD is dedicated to teaching in the classroom and the laboratory. She strives to keep students motivated and engaged by relating course material to real-world problems or other disciplines. Dr. Park envisions utilizing these methods to teach microbiology, biology, biochemistry and seminars coursework.

RESEARCH INTERESTS:

Biosynthetic pathways of natural products from microorganisms with the ultimate goal of finding promising novel drug candidates

STUDENT RESEARCH OPPORTUNITIES:

Sung Ryeol Park, PhD’s laboratory will characterize biosynthetic pathways of microbial secondary metabolites and the variety of enzyme classes responsible for their production. Microbial fermentation process will enable students to contribute to the screening and optimization of biologically active molecules. This research will provide students with professional experiences and a deep understanding of the microbial genetics, bioinformatics, biochemistry and enzymology involved in microbial natural product biosynthesis.


PUBLICATIONS:

36.

Yoo, Y.J., Kim, H. S., Park, S.R.*, Yoon, Y.J.* (2016) An overview of rapamycin: from discovery to future perspectives. J. Ind. Microbiol. Biotechnol. DOI 10.1007/s10295-016-1834-7. Review. *co-corresponding authors.

35.

DeMars, M.D. II , Sheng, F., Park, S.R., Lowell, A.N., Podust, L.M., Sherman, D.H. (2016) Biochemical and Structural Characterization of MycCI, a Versatile P450 Biocatalyst from the Mycinamicin Biosynthetic Pathway. ACS Chem. Biol. 11:2642-2654

34.

Ban, Y.H.*, Park, S.R.*, and Yoon, Y.J. (2016) A review of the bioactivity, biosynthesis and genetic engineering of FK506: from the past achievements to the future prospects. J. Ind. Microbiol. Biotechnol. 43: 389-400. Review. *co-first authors.

33.

Park, S.R.*, Tripathi, A.*, Wu, J., Schultz, P.J., Yim, I., Yu, F., Arevang, C., Tamayo-Castillo, G., Xi, C., and Sherman, D.H., (2016) Discovery of cahuitamycins as biofilm inhibitors derived from a convergent biosynthetic pathway. Nat. Commun. 7:10710. *co-first authors.

32.

Almutairi, M., Park, S.R., Hansen, D.A., Vázquez-Laslop N, Douthwaite, S, Sherman, D.H. and Mankin, A.S. (2015) The mode of action and regulation of the resistance genes from the producer of natural ketolide antibiotics. Proc. Natl. Acad. Sci. USA. 112: 12956-12961.

31.

Chemler, J, Tripathi, A., Hansen, D., O’Neil-Johnson, M., Williams, R., Starks, C, Park, S.R., Sherman, D.H., (2015) Evolution of efficient modular polyketide synthases by homeologous recombination. J. Am. Chem. Soc. 137: 10603-10609.

30.

Park, S.R., and Yoon, Y.J. (2015) Combinatorial biosynthesis for antibiotic discovery. Antibiotics:Current Innovations and Future Trends. Book chapter. Caister Academic Press. Chapter 16:293-319.

29.

Bernard, S.M., Akey, D.L., Tripathi, A., Park, S.R., Konwerski, J.R., Anzai, Y., Li, S., Kato, F., Sherman, D.H., and Smith, J.L. (2015) Structural basis of substrate specificity and regiochemistry in the MycF/TylF family of sugar O-methyltransferases. ACS Chem. Biol. 10: 1340-1351.

28.

Song, M.C., Kim, E., Ban, Y.H., Yoo, Y.J., Kim, E.J., Park, S.R., Pandey, R.P., Sohng, J.K., and Yoon, Y.J. (2013) Achievements and impacts of glycosylation reactions involved in natural product biosynthesis in prokaryotes. Appl. Microbiol. Biotechnol. 97:5691-5704.

27.

Park, S.R., Park, J.W., Ban, Y.H., Sohng, J.K., and Yoon, Y.J. (2013) 2-Deoxystreptamine-containing aminoglycoside antibiotics: Recent advances in the characterization and manipulation of their biosynthetic pathways. Nat. Prod. Rep. 30: 11-20. Review.

26.

Han, A.R., Shinde, P.B., Park, J.W., Cho, J., Lee, S.R., Ban, Y.H., Yoo, Y.J., Kim, E.J., Kim, E., Park, S.R., Kim, B-G., Lee, D.G., and Yoon, Y.J. (2012) Engineered biosynthesis of glycosylated derivatives of narbomycin and evaluation of their antibacterial activities. Appl. Microbiol. Biotechnol. 93:1147-1156.

25.

Park, S.R., Ahn, M.S., Han, A.R., Park, J.W., and Yoon, Y.J. (2011) Enhanced flavonoid production in Streptomyces venezuelae via metabolic engineering. J. Microbiol. Biotechnol. 21:1143-1146.

24.

Park, J.W.*, Park, S.R.*, Nepal, K., Han, A.R., Ban, Y.H., Yoo, Y.J., Kim, E.J., Kim, E.M., Kim, D., Sohng, J.K., and Yoon, Y.J. (2011) Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation. Nat. Chem. Biol. 7:843-852. *co-first authors.

23.

Han, A.R., Park, S.R., Park, J.W., Lee, E.Y., Kim, D-M., Kim, B.G., and Yoon, Y.J. (2011) Biosynthesis of glycosylated derivatives of tylosin in Streptomyces venezuelae. J. Microbiol. Biotechnol. 21:613-616.

22.

Jung, W.S., Yoo, Y.J., Park, J.W., Park, S.R., Han, A.R., Ban, Y.H., Kim, E.J., Kim, E., and Yoon, Y.J. (2011) A combined approach of classical mutagenesis and rational metabolic engineering improves rapamycin biosynthesis and provides insights into methylmalonyl-CoA precursor supply pathway in Streptomyces hygroscopicus ATCC 29253. Appl. Microbiol. Biotechnol. 91:1389-1397.

21.

Park, J.W., Park, S.R., Han, A.R., Ban, Y.H., Yoo, Y.J., Kim, E.J., and Yoon, Y.J. (2011) Microbial transformation of trichostatin A to 2,3-dihydrotrichostatin A. J. Nat. Prod. 74:1272-1274.

20.

Park, J.W., Park, S.R., Han, A.R., Ban, Y.H., Yoo, Y.J., Kim, E.J., Kim, B.S., Sohng, J.K., and Yoon, Y.J. (2011) Characterization and identification of pradimicin analogs from Actinomadura hibisca using liquid chromatography-tandem mass spectrometry. J. Chromatography A 1218:2284-2291.

19.

Park, J.W., Park, S.R., Han, A.R., Ban, Y.H., Yoo, Y.J., Kim, E.J., Kim, E., Sohng, J.K., and Yoon, Y.J. (2011) Generation of reduced macrolide analogs by regio-specific biotransformation. J. Antibiot. 64:155-157.

18.

Mo, S.J., Kim, D.H., Lee, J.H., Park, J.W., Basnet, D.B., Ban, Y.H., Yoo, Y.J., Chen, S-W., Park, S.R., Ghoi, E.A., Kim, E., Jin, Y-Y., Lee, S-K., Park, J.Y., Liu, Y., Lee, M.O., Lee, K.S., Kim, S.J., Kim, D., Park, B.C., Lee, S.-G., Kwon, H.J., Suh, J.-W., Moore, B.S., Lim, S.-K., and Yoon, Y.J. (2011) Biosynthesis of the allylmalonyl-CoA extender unit for the FK506 polyketide synthase proceeds through a dedicated polyketide synthase and facilitates the mutasynthesis of analogues. J. Am. Soc. Chem. 133: 976-985.

17.

Park, S.R., Paik, J.H., Ahn, M.S., Park, J.W., and Yoon, Y.J. (2010) Biosynthesis of plant-specific flavones and flavonols in Streptomyces venezuelae. J. Microbiol. Biotechnol. 20: 1295-1299.

16.

Park, S.R., Yoo, Y.J., Ban, Y.H., and Yoon, Y.J. (2010) Biosynthesis of rapamycin and its regulation: past achievements and recent progress. J. Antibiot. 63: 434-441. Review.

15.

Park, J.W., Park, S.R., Han, A.R., Ban, Y.H., Yoo, Y.J., Kim, E.J., and Yoon, Y.J. (2010) The nebramycin aminoglycoside profiles of Streptomyces tenebrarius and their characterization using an integrated liquid chromatography-electrospray ionization-tandem mass spectrometric analysis. Anal. Chim. Acta. 661: 76-84.

14.

Park, S.R., Han, A.R., Ban, Y.H., Yoo, Y.J., Kim, E.J., and Yoon, Y.J. (2010) Genetic engineering of macrolide biosynthesis: past advances, current state, and future prospects. Appl. Microbiol. Biotechnol. 85: 1227-1239. Review.

13.

Park, J.W., Mo, S.J., Park, S.R., Ban, Y.H., Yoo, Y.J., and Yoon, Y.J. (2009) Liquid chromatography-mass spectrometry characterization of FK506 biosynthetic intermediates in Streptomyces clavuligerus KCTC 10561BP. Anal. Biochem. 393: 1-7.

12.

Park, S.R., Yoon, J.A., Paik, J.H., Park, J.W., Jung, W.S., Ban, Y.H., Kim, E.J., Yoo, Y.J., Han, A.H., and Yoon, Y.J. (2009) Engineering of plant-specific phenylpropanoids biosynthesis in Streptomyces venezuelae. J. Biotechnol. 141: 181-188.

11.

Hwang, G., Park, S.R., Lee, C.H., Ahn, I.S., Yoon, Y.J., and Mhin, B.J. (2009) Influence of naphthalene biodegradation on the adhesion of Pseudomonas putida NCIB 9816-4 to a naphthalene-contaminated soil. J. Hazard Mater. 172:491-493.

10.

Park, J.W., Oh, H.S., Jung, W.S., Park, S.R., Han, A.R., Ban, Y.H., Kim, E.J., Kang, H.Y., and Yoon, Y.J. (2008) Exploiting the natural metabolic diversity of Streptomyces venezuelae to generate unusual reduced macrolides. Chem. Comm. 30:5782-5784.

9.

Park, S.R., Park, J.W., Jung, W.S., Han, A.R., Ban, Y.H., Kim, E.J., Shong, J.K., Sim, S.J., and Yoon, Y.J. (2008) Heterologous production of epothilones B and D in Streptomyces venezuelae. Appl. Microbiol. Biotechnol. 81:109-117.

8.

Park, J.W., Hong, J.S.J., Parajuli, N., Jung, W.S., Park, S.R., Lim, S.K., Sohng, J.K., and Yoon, Y.J. (2008) Genetic dissection of the biosynthetic route to gentamicin A2 by heterologous expression of its minimal gene set. Proc. Natl. Acad. Sci. USA. 105: 8399-8404.

7.

Jung, W.S., Jeong, S.J., Park, S.R., Choi, C.Y., Park, B.C., Park, J.W., and Yoon, Y.J. (2008) Enhanced heterologous production of desosaminyl macrolides and their hydroxylated derivatives by overexpression of pikD regulatory factor in Streptomyces venezuelae. Appl. Environ. Microbiol. 74:1972-1979.

6.

Jung, W.S., Han, A.R., Hong, J.S.J., Park, S.R., Choi, C.Y., Park, J.W., and Yoon, Y.J. (2007) Bioconversion of 12-, 14-, and 16-membered ring aglycones to glycosylated macrolides in an engineered strain of Streptomyces venezuelae. Appl. Microbiol. Biotechnol. 76:1373-1381.

5.

Park, J.W., Jung, W.S., Park, S.R., Park, B.C., and Yoon, Y.J. (2007) Analysis of intracellular short organic acid-coenzyme A esters from actinomycetes using liquid chromatography-electrospray ionization-mass spectrometry. J. Mass Spec. 42:1136-1147.

4.

Park, J.W., Hong, J.S.J., Niranjan, P., Koh, W.S., Park, S.R., Lee, M.I., Lim, S.K., and Yoon, Y.J. (2007) Analytical profiling of biosynthetic intermediates involved in the gentamicin pathway of Micromonospora echinospora by high-performance liquid chromatography using electrospray ionization mass spectrometric detection. Anal. Chem. 79: 4860-4869.

3.

Hong, J.S.J., Park, S.J., Parajuli, N., Park, S.R., Koh, H.S., Jung, W.S., Choi, C.Y., and Yoon, Y.J. (2007) Functional analysis of DesVIII homologues involved in glycosylation of macrolide antibiotics by interspecies complementation. Gene 386:123-130.

2.

Jung, W.S., Lee, S.K., Hong, J.S.J., Park, S.R., Jeong, S.J., Han, A.R., Sohng, J.K., Kim, B.G., Choi, C.Y., Sherman, D.H., and Yoon, Y.J. (2006) Heterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae. Appl. Microbiol. Biotechnol. 72:763-769.

1.

Lee, S.K., Park, J.W., Kim, J.W., Jung, W.S., Park, S.R., Choi, C.Y., Kim, E.S., Kim, B.S., Ahn, J.S., Sherman, D.H., and Yoon, Y.J. (2006) Neopikromycin and novapikromycin from the pikromycin biosynthetic pathway of Streptomyces venezuelae. J. Nat. Prod. 69:847-849.