Molecular Biology & Protein Science Core Facility

The facility offers:

  • Consultations on experimental design and data validation
  • RNA, DNA, protein isolation and purification
  • Protein separation (2-D gels, HPLC, FPLC)
  • Circular dichroism
  • Biacore surface plasmon resonance
  • Quantitative real-time PCR analysis ( RNA, DNA, SNP)
  • Data analysis

Equipment in the Molecular Biology & Protein Science Core

  • Applied Biosystems 7900HT Fast real time PCR machine (96/384 wells)
  • Applied Biosystems 7500 real time PCR machine (96 well)
  • MagMAX™ Express-96 standard magnetic particle processor
  • 7900 low density Array micro fluidic card system
  • Applied Biosystems Veriti thermal cycler
  • (6) Applied Biosystems 2720 thermal cyclers
  • (4) Dell computers
  • Turner biosystems luminometer
  • Molecular devices M2 microplate reader
  • Molecular devices M5 microplate reader
  • (2) Alpha Innotech FluroChem HD2 digital gel imaging systems
  • (2) Beckman Coulter DU 700 series UV/Vis spectophotometer
  • (2) Source One X-ray developers
  • Beckman Coulter Z series Coulter counter
  • Nucleofector II Device (Amaxa)
  • Electrophoresis units for DNA sequencing
  • Agarose gel electrophoresis
  • Power units
  • Orbital shakers
  • UV illuminator with Polariod camera, X-ray absorptiometry (Faxitron systems)

For gene analysis:

  • Applied Biosystems 7900HT Fast real time PCR Machine (96/384 wells)
  • Applied Biosystems 7500 real time PCR Machine (96 well)
  • MagMAX™ Express-96 Standard Magnetic Particle Processor
  • 7900 low Density Array micro fluidic card system
  • Applied Biosystems Veriti Thermal Cycler,
  • (6) Applied Biosystems 2720 Thermal Cyclers
  • Turner Biosystems Luminometer
  • Molecular Devices M2 Microplate Reader
  • Molecular Devices M5 Microplate Reader
  • (2) Alpha Innotech FluroChem HD2 Digital gel imaging systems
  • (2) Beckman Coulter DU 700 series UV/Vis Spectophotometer
  • (2)Source One X-ray Developers
  • Beckman Coulter Z series Coulter Counter
  • Nucleofector II Device (Amaxa)
  • Electrophoresis units for DNA sequencing
  • Agarose gel electrophoresis
  • Power units
  • Orbital shakers
  • UV illuminator with Polariod camera

For protein analysis:

  • Biacore (Surface Plasmon Resonance)
  • Waters HPLC system with built in degasser and Autosampler, which has UV/vis dual wavelength detector, fluorescence detector, and electrochemical detector. The HPLC is operated with Empower2 software (Waters) for data collection and analysis which is installed on Dell PC, FPLC and chromatography cabinets
  • FPLC
  • Clark Type oxygen electrode (YSI) connected to IBM computer that runs data acquisition software
  • 2-D and 1-D electrophoresis systems
  • DU730 spectrophotometer
  • SRX101A Processor
  • FluorChem HD2 system
  • 600 watt digital sonicator
  • Alpha Innotech
  • Alpha-Imager digital documentation systems for gel visualization and documentation

Microarray and proteomics:

Microarray analysis and Protein identification by Mass Spectrometry are kindly provided by our friends at Penn State Hershey and Rhode Island Hospital CoBRE for Cancer Research Development. If you access these cores, please remember to communicate to them any publications or grant applications funded, for their records.

Additionally the facility contains Dell computers, refrigerators, -20ºC and -80ºC freezers, and associated small equipment (e.g. multichannel, electronic, and accordion pipettes).

Services of the Molecular Biology & Protein Science Core:

  • Real time PCR
  • PCR amplification of DNA
  • RNA
  • Digital imaging
  • Cell counting
  • Cell transfections
  • DNA electrophoresis
  • DNA quantification
  • Viacore (Surface Plasmon Resonance)
  • Circular Dichorism
  • FPLC
  • HPLC
  • 2-D electrophoresis
  • Protein purification
  • X-ray crystallography

Location

Geisinger Commonwealth School of Medicine
Medical Sciences Building
525 Pine Street
Scranton, PA 18503

Citing the Core in Publications

When using data or tools generated at Geisinger Commonwealth in publications or grants, please cite the Core Laboratory personnel who worked on the project and the “Molecular Biology & Protein Science Core Facility, Geisinger Commonwealth School of Medicine” as appropriate.

Please also let research leadership know about publications or grant applications that make use of the services of this facility – this is important for continued availability of these services.

Rates and Time Scheduling

Rates for equipment and Core Facility usage are priced competitively. To obtain rates or schedule time in the Core, please contact the Molecular Biology & Protein Analysis Core director, Shagufta Khan.

Molecular Biology & Protein Science Core Contact

Shagufta Khan: skhan@tcmc.edu